2 edition of Ultrastructure of Xenopus laevis foreimb regenerates: influence of limb denervation. found in the catalog.
Ultrastructure of Xenopus laevis foreimb regenerates: influence of limb denervation.
Paul Allen Khan
Written in English
|The Physical Object|
|Number of Pages||100|
Insulin Localization in the Pancreas and in Forelimb Regenerates of the Adult Newt Notophthalmus viridescens.- Lens and Limb Regeneration in Amphibia Under the Spaceflight Conditions.- In Vitro Effects of a Mitogenic Factor on Xenopus laevis Forelimb Regeneration.- Commentary.- Muscle.- Some Aspects of Regeneration in Skeletal and Cardiac Muscle Interestingly, studies on limb, retina and nerve tissue regeneration in Xenopus laevis show that these structures can fully regenerate in tadpoles although they cannot regenerate during adult life.
After denervation, postamputation formation of the regeneration blastema does not occur in early Xenopus larvae, but it occurs in late larvae. The reason for the difference is the fact that fgf2 mRNA, which is released by local nerves (Mullen et al., ) in early larvae, is present in high levels, while in late larvae it is low (Cannata. Amputation of the limb triggers the breakdown of extracellular matrices (ECMs) at the wounded sites and the nearby tissues. ECM exerts a profound influence on a variety of biologically important phenomena such as growth, differentiation, migration, apoptosis, and maturation of cells in animal tissues (Hay, ).
Singer M: Induction of regeneration of the forelimb of the post-metamorphic frog by augmentation of the nerve supply. J Exp Zool –, 8. Singer M, Kamrin RP, Ashbaugh A: The influence of denervation upon trauma induced regenerates of the forelimb of the post-metamorphic frog. J Exp Zool –51, In situ hybridization chain reaction probing for vwde in (a–f) regeneration‐competent Xenopus laevis tails (a,b) before amputation, (c,d) blastema 24 hr postamputation, and (e,f) the peripheral edge of the amputation plane 24 hr postamputation. (g–l) Regeneration‐refractory tails (g,h) before amputation, (i,j) blastema 24 hr.
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Xenopus laevis froglet forelimbs normally respond to amputational injury by forming a heteromorphic cartilaginous rod-shaped outgrowth. However, partial denervation of a forelimb. Summary. This study concerns the effects of nerve augmentation on growth and development of forelimb regenerates in metamorphosed Xenopus laevis froglets.
Augmentation was accomplished by implanting spinal ganglia from sibling donors near the base of the host by: 4. Experiments were designed to test adaptability of forelimb spike regenerates in Xenopus laevis froglets.
The results show that when amputation is at the radius/ulna level, regeneration occurs in. As studied in larval Xenopus laevis, once differentiation begins in cell of developing hindlimbs, regeneration after amputation is normally incomplete and the ontogenetic decline in limb.
When a X. laevis froglet forelimb is denervated 1 -3 weeks (Korneluk et al. ) or 3 -8 weeks (Kurabuchi and Inoue ) after amputation, the regenerates undergo partial or. The effects of denervation on limb regeneration of postmetamorphic Xenopus laevis in the early to late stages of regeneration were studied.
Limbs that. An Overview of the Historical Origin of the Nerve Influence on Limb Regeneration. In Vitro Effects of a Mitogenic Factor on Xenopus Laevis Forelimb Regeneration.
Catherine Tsilfidis, Richard A. Liversage. Pages About this book. Introduction. Filoni S, Velloso C P, Bernardini S and Cannata S M Acquisition of nerve dependence for the formation of a regeneration blastema in amputated hindlimbs of Xenopus laevis: the role of limb innervation and that of limb differentiation J.
Exp. Zool. – We studied the effect of denervation on the extracellular matrix of a newt limb regenerate with a specific dye of collagen, Sirius red.
Blastemata harvested on adult animals 4 days after denervation accumulated more collagen (+54%) than blastemata harvested just after denervation.
Introduction. The Xenopus tadpole is able to regenerate its tail, including restoration of its nerve, muscle, skin and blood vessel components (Deuchar, ).This robust and rapid ability to regenerate the morphology of important tissues has drawn considerable interest as a tractable model for the investigation of this biomedically important phenomenon.
The removal of the neural tube in salamander embryos allows the development of nerve-free aneurogenic limbs. Limb regeneration is normally nerve-dependent, but the aneurogenic limb regenerates without nerves and becomes nerve-dependent after innervation. The molecular basis for these tissue interactions is unclear.
Anterior Gradient (AG) protein, previously shown to rescue regeneration. Briegleb W, Neubert J, Schatz A, Klein T, Kruse B.
Survey of the vestibulum, and behavior of Xenopus laevis larvae developed during a 7-days space flight. Adv Space Res.
; – Bryant SV, Fyfe D, Singer M. The effects of denervation on the ultrastructure of young limb regenerates in the newt, Triturus. Dev Biol. ; – INTRODUCTION. Urodele amphibians are unique in being able to regenerate amputated limbs at both larval and adult stages of the life cycle.
Important to limb regeneration is the formation of a blastema, a mass of undifferentiated mesenchyme cells covered by a multilayered epithelium (Wallace, ; Stocum, ; Tsonis, ).This epithelium is derived from skin epidermis within hours of limb.
Filoni S, Bernardini S, Cannata SM () The influence of denervation on grafted hindlimb regeneration of larval Xenopus laevis. J exp Zool – PubMed CrossRef Google Scholar Filoni S, Bernardini S, Cannata SM, Ghittoni R () Nerve-independence of limb regeneration in larval Xenopus laevis is related to the presence of mitogenic.
Regenerating tissue of the newt forelimb extensively incorporates radioactive phosphate into the H2A histone subfraction, with little or no apparent l. The AEC of the accumulation blastema is invaded by sprouting sensory axons as it forms, whereas other sensory axons and motor axons make intimate contact with mesenchyme cells of the dent with innervation of the AEC, the labeling and mitotic indices of the accumulation blastema rise as much as fold and the blastema enters the phase of.
An Overview of the Historical Origin of the Nerve Influence on Limb Regeneration --Gene Expression --Identification of a Homeobox Gene and a Keratin Pair Expressed in Amphibian Limb Book\/a> ; \u00A0\u00A0\u00A0\n library:oclcnum\/a In Vitro Effects of a Mitogenic Factor on Xenopus laevis Forelimb Regeneration.
It is unlikely that this relates to the failure of limb regeneration in Xenopus, which occurs in a proximal-to-distal direction beginning at stage We observed that the down-regulation of AG expression does not begin, even in proximal regions, until the prometamorphic stages 58–59 (Fig.
S2 A – C). Fgf8 is associated with anterior patterning of the blastema during limb regeneration in Xenopus (Christen & Slack, ). Transfection of shh into anterior blastema tissue of the axolotl limb by vaccinia virus results in the regeneration of supernumerary digits, but not more proximal structures (Roy, Gardiner, & Bryant, ).
David Deck, Morphological effects of partial denervation on regeneration of the larval salamander forelimb, Journal of Experimental Zoology, /jez,3, (), (). Wiley Online Library. The relative contributions of innervation and androgen to three muscle fiber properties—twitch type, size, and number—were examined in the sexually dimorphic, androgen-sensitive laryngeal muscle of Xenopus laevis.
In adults, the muscle contains all fast-twitch fibers in males and fast- and slow-twitch fibers in females; laryngeal muscle fibers are larger and more numerous in .Microarray analysis and cDNA sequencing were used to investigate a centuries-old problem in regenerative biology: the basis of nerve-dependent limb regeneration in salamanders.
Innervated (NR) and denervated (DL) forelimbs of Mexican axolotls were amputated and transcripts were sampled after 0, 5, and 14 days of regeneration.
Considerable similarity was .Xenopus laevis without any thyroid hormone influence confirms that the myogenic program in adult muscle regeneration is regulated by factors that are different from those regulating normal development. The aim of the present work was to study the ultrastructure of the newly differentiated muscles of the regenerating limb of a larval stage of.